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pcr-select  (TaKaRa)


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    Structured Review

    TaKaRa pcr-select
    Pcr Select, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacterial+genome+subtraction+kit/Clontech+PCR-Select+Bacterial+Genome+Subtraction+Kit/pmc00116598-122-8-7
    Average 95 stars, based on 182 article reviews
    pcr-select - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa
    Article Snippet: .. The Clontech PCR-Select Bacterial Genome Subtraction kit was used to generate the SSH library of Rsa I fragments according to manufacturer's instructions but with a hybridization temperature of 73°C. .. The library of SSH PCR amplicons was cloned in pGEM-T Easy (Promega).

    Article Title: Subtracted Diversity Array Identifies Novel Molecular Markers Including Retrotransposons for Fingerprinting Echinacea Species
    Article Snippet: .. Subtraction was performed using the PCR-SelectTM Bacterial Genome Subtraction Kit (Clontech), following the manufacturer’s protocol. ..

    Article Title: Efficient Anchoring of Erianthus arundinaceus Chromatin Introgressed into Sugarcane by Specific Molecular Markers
    Article Snippet: .. SSH was carried out as described in instructions for the Clontech PCR-Select Bacterial Genome Subtraction Kit (Clontech, Mountain View, CA, USA). ..

    Article Title: Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
    Article Snippet: .. SSH was carried out between APEC strain IMT5155 and UPEC strain CFT073 using Clontech PCR-SelectTM Bacterial Genome Subtraction Kit (Clontech, Heidelberg, Germany) according to the manufacturer's manual. ..

    Article Title: Efficient Anchoring of Erianthus arundinaceus Chromatin Introgressed into Sugarcane by Specific Molecular Markers
    Article Snippet: .. SSH was carried out as described in instructions for the Clontech PCR-Select Bacterial Genome Subtraction Kit (Clontech, Mountain View, CA, USA). ..

    Article Title: Use of suppression subtractive hybridisation to extend our knowledge of genome diversity in Campylobacter jejuni
    Article Snippet: .. SSH was carried out using the CLONTECH PCR-SelectTM Bacterial Genome Subtraction Kit (Clontech) essentially as recommended by the supplier but with a hybridisation temperature of 58°C. ..

    Article Title: Development of a Strain-Specific Real-Time PCR Assay for Enumeration of a Probiotic Lactobacillus reuteri in Chicken Feed and Intestine
    Article Snippet: Isolated DNA was visualized by agarose gel electrophoresis and concentration was determined by NanoDrop ND-1000 spectrophotometer (Peqlab Biotechnology GmbH, Erlangen, Germany). .. For SSH, the PCR-select bacterial genome subtraction kit (Clonetech Laboratories, Mountain View, CA, USA) was used to subtract unique genomic DNA of the probiotic strain L. reuteri LR (tester DNA) from the type strain L. reuteri 20016 T (driver DNA) following the manufacturer's protocol with modifications. .. The first hybridization step was performed at 55°C for 90 min and the second hybridization at 55°C for 16 h. Primary and secondary (nested) PCR were conducted using the Advantage 2 Polymerase Mix (Clonetech Laboratories, Mountain View, CA, USA), 10 mM dNTP mix, 10 μM of each primer, and 1 μl template DNA.

    Article Title: In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae , African strain MAI1
    Article Snippet: The DNA was transferred to Hybond-N + nylon membranes (Amersham Pharmacia Biotech) by capillary transfer. .. The insert DNA was amplified by PCR, using the nested primers provided with the PCR-SelectTM Bacterial Genome Subtraction Kit (Clontech Laboratories, Inc.). .. The amplified DNA fragment was gel purified, using the QIAquick Gel Extraction Kit (QIAGEN, Inc.), as recommended by the manufacturer.

    Hybridization:

    Article Title: A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa
    Article Snippet: .. The Clontech PCR-Select Bacterial Genome Subtraction kit was used to generate the SSH library of Rsa I fragments according to manufacturer's instructions but with a hybridization temperature of 73°C. .. The library of SSH PCR amplicons was cloned in pGEM-T Easy (Promega).

    Article Title: Use of suppression subtractive hybridisation to extend our knowledge of genome diversity in Campylobacter jejuni
    Article Snippet: .. SSH was carried out using the CLONTECH PCR-SelectTM Bacterial Genome Subtraction Kit (Clontech) essentially as recommended by the supplier but with a hybridisation temperature of 58°C. ..

    Amplification:

    Article Title: In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae , African strain MAI1
    Article Snippet: The DNA was transferred to Hybond-N + nylon membranes (Amersham Pharmacia Biotech) by capillary transfer. .. The insert DNA was amplified by PCR, using the nested primers provided with the PCR-SelectTM Bacterial Genome Subtraction Kit (Clontech Laboratories, Inc.). .. The amplified DNA fragment was gel purified, using the QIAquick Gel Extraction Kit (QIAGEN, Inc.), as recommended by the manufacturer.



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